Medically assisted reproduction
Laboratory Procedures
Laboratory Procedures
Laboratory Procedures
Clean room technology
By equipping the new laboratory, every detail was carefully selected to increase the chances of pregnancy.
- The laboratory is isolated from all other spaces with an airlock.
- The walls are made of antibacterial panels used in clean room technology.
- The paints and adhesives used in construction are selected to contain minimal amounts of toxic substances.
Advantages of clean room technology compared to conventional IVF laboratories
With all these modern techniques, we provide the best possible environment for the success of the embryo.
- The laboratory premises are heated and the air in them is continuously filtered for 8 weeks (the so-called burn out), removing all possible traces of toxic compounds
- Three series of external filters and one internal filter clean the air from dust, smog, spores, bacteria, viruses and organic substances
- Purified air under a pressure of 20 Ps is introduced into the laboratory with 20-30 air changes per hour
- The rooms next to the laboratory have a lower pressure of purified air so that the air always leaves the laboratory
MACS (magnetic cell sorting)
is a new method of sperm selection using magnetic cell sorting. Defective but morphologically unrecognizable cells are selectively removed from the patient's sample. A high degree of DNA fragmentation is one of the invisible signs of sperm deterioration, which can negatively affect the arrest of development at the blastocyst stage or lead to early abortion. Using the MACS method:
- Damaged sperm can be identified and isolated very early
- We increase the success of fertilization and the quality of embryos in in vitro fertilization
The HBA test and PICSI is a method
that determines the maturity of the sperm in the original semen sample (before any treatment that separates the sperm with the best characteristics).
The test is based on the ability of mature sperm to bind to hyaluronan, a compound found in high concentrations in the follicular fluid in which the egg develops, and the cumulus oophorus, a group of cells and intercellular substance that surrounds the egg.
Sperm that bind to hyaluronans:
- They have better morphology and 4-6 times less chromosomal aneuploidy
- They have a higher chance of successful fertilization
- The combination of two methods (MAC and PICSI) gives excellent results
Vitrification is a method of storing eggs
and embryos in which the sample is immersed directly in liquid nitrogen at -196°C, which leads to a "glaze" of the liquid part of the cell, the so-called. vitrification.
- With the introduction of this method of cryopreservation, the survival of egg cells increased to 90-100%.
Halosperm is a DNA fragmentation test
Sperm DNA fragmentation is one of the first signs of sperm deterioration and cannot be correlated with common semen analysis parameters such as sperm count, concentration and motility.
This test is extremely useful and helps answer the following questions:
- which couples are suitable for AIH (Insemination)
- whether a modified approach (ICSI, PICSI, IMSI) should be applied to IVF.
- whether the varicocele surgery was effective
- how much damage is caused by infection with chlamydia and mycoplasma
- which is why the failure of MPO procedures is repeated in some couples
IMSI procedure
From the very beginning of the treatment of male infertility with micromanipulation methods, one of the main problems is the choice of sperm. In order to prevent the selection of potentially unsuitable sperm for fertilization and proper embryonic development, we have developed modified variants of ICSI called IMSI (intracytoplasmic morphologically selected sperm injection) by which we select the morphologically best sperm.
The combination of PICSI and IMSI method gives the best results in cases of marital infertility caused by the male factor.
The ZyMōt multiple sperm separation device
prepares motile sperm from semen for use in intrauterine insemination (IUI) and intracytoplasmic sperm injection (ICSI) procedures.
ZyMōt devices separate sperm based on motility, without the need for centrifugation.
EmbrioGlue
is a medium that closely resembles the environment in the uterus at the time of implantation. Placing the embryo in EmbryoGlue prior to transfer gives it an even better chance of implantation.
Thanks to a team of top embryologists and following new technological developments, the Cito embryology laboratory is equipped with "state of the art" devices for the treatment of even the most complicated cases of infertility, and thus a higher rate of successful pregnancies resulting from medical insemination.
Our Happy Patients
Our Expert Doctos
Petar Poljak, MD
specialist in gynecology and obstetrics
subspecialist in gynecological endocrinology and human reproduction
dr.petar.poljak@cito.hr
Doc.Dr.sc. Nađa Aračić, MD
specialist in gynecology and obstetrics
subspecialist in gynecological endocrinology and human reproduction
dr.aracic@cito.hr
Primarius mr.sc. Boris Poljak, MD
specialist in Gynecology and obstetrics
Subspecialist in Gynecological endocrinology and human reproduction
dr.poljak@cito.hr
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