Medically Assisted Reproduction
Medically Assisted Reproduction
Since 2006,
the Polyclinic has had the most equipped and most successful laboratory for medically assisted fertilization in the region, fully equipped to perform all methods of in vitro fertilization.
3178
BABIES BORN FROM IVF
7566
IVF PROCEDURES
15
YEARS OF EXP IN MAR
The first medically assisted fertilization in the region was performed in 2001 by primarius Boris Poljak, MD (founder and director of Polyblinic CITO) and mr.sc. Anamarija Baranović, B.Sc. biology
Our IVF lab is lead by Dr.sc. Pavle Romac, B.Sc. of Biology, Mr.sc. Anamarija Baranović, B.Sc. biology & Lucija Romac, B.Sc. biology
MAR Includes
Quick Filters
Today, it is estimated that about 15% have fertility problems in one or both partners. According to the instructions of the WHO (World Health Organization), if after one year of trying the desired pregnancy did not occur, you should inform your doctor for a consultation and preliminary tests
- Cervical swabs
- Induction of ovulation
- Hormonal status
- 3D ultrasound of uterus
- Spermiogram
- Insemintaion (AIH)
- Ultrasound 3D folliculometry
- Hysterosalpingo-foam sonography (HyFoSy)
Thanks to experience and equipment, we can treat even the most complicated cases of infertility in the polyclinic. Our first goal is to determine why you are not pregnant and then help you find the best and fastest solution to your problem. Every infertility is unique and should be approached that way.
How IVF works?
From the beginning of infertility treatment, it is important to solve the problem in a certain order, preferably diagnose the cause, and then treat it with the optimal method.
STEP 1
Recognize problem
If you do not get pregnant after having unprotected sex for 1 year. If you are older than 35 or if you do not get your p...
If you do not get pregnant after having unprotected sex for 1 year. If you are older than 35 or if you do not get your period every month, see your gynaecologist if you can’t get pregnant after 6 months of trying.
STEP 2
Consultations
From the beginning of infertility treatment, it is important to solve the problem in a certain order, preferably diagnos...
From the beginning of infertility treatment, it is important to solve the problem in a certain order, preferably diagnose the cause, and then treat it with the optimal method. Spermiogram, microbiological tests, gynecological and ultrasound examination are the basics from which to start.
STEP 3
Decision to go for IVF
After thorough preparations, when all the simpler ways of infertility therapy have been tried and it is established that...
After thorough preparations, when all the simpler ways of infertility therapy have been tried and it is established that in vitro fertilization is the only way you can get pregnant and give birth to a healthy child, we plan an appropriate assisted fertilization procedure. The procedure consists of the following stages.
STEP 4
Hormone stimulation
Based on the results of the diagnosis and basal values of pituitary hormones (2nd – 4th day of the cycle – F...
Based on the results of the diagnosis and basal values of pituitary hormones (2nd – 4th day of the cycle – FSH, LH, PRL), a stimulation protocol will be determined that will give the optimal result in your case.
STEP 5
Egg pickup
After the growth and maturation of the follicles, the follicular fluid in which the eggs are located is taken (aspirated...
After the growth and maturation of the follicles, the follicular fluid in which the eggs are located is taken (aspirated) by transvaginal puncture. In the IVF laboratory, the contents of the follicles are examined under a magnifying glass and eggs are isolated.
STEP 6
Fertilization
On the day of aspiration the partner gives semen which is processed and prepared for one of the IVF procedures. Dependin...
On the day of aspiration the partner gives semen which is processed and prepared for one of the IVF procedures. Depending on the finding, fertilization will be attempted by insemination of ova (in case of normozoospermia) or by ICSI (intracytoplasmic sperm injection) method in which sperm is selected, activated and inserted into a ovum previously prepared for this procedure with a glass micropipette.
STEP 7
Embriotransfer
On the second or fifth day after the puncture, depending on the number and quality of embryos, embryo transfer of one or...
On the second or fifth day after the puncture, depending on the number and quality of embryos, embryo transfer of one or more embryos is performed. How many embryos will return to the uterus depends on their quality, the number of previous attempts and the patient’s health.
Infertility in a couple is defined as the inability to achieve conception despite one year of frequent, unprotected intercourse. In approximately 35 percent of couples with infertility, a male factor is identified along with a female factor.
When analyzing male fertility (spermiogram), among other things, we are able to make the following tests, analyzes and procedures.
Spermiogram
Spermiogram is an ejaculate analysis that determines the total number, concentration, mobility and appearance (morpholog...
Spermiogram is an ejaculate analysis that determines the total number, concentration, mobility and appearance (morphology) of sperm. In the diagnosis of male infertility, this is the basic examination on the basis of which the further course of action in the treatment of the infertility couple is decided.
More about spermiogram
SCA morphology
Is a program that allows automatic morphometric analysis and classification of sperm according to …
Is a program that allows automatic morphometric analysis and classification of sperm according to …
Link to the news
Halosperm – DNA fragmentation test
To determine which pairs are suitable for AIH, whether a modified approach (PICSI…
To determine which pairs are suitable for AIH, whether a modified approach (PICSI…
Link to the news
HBA Test
This test determines the maturity of the sperm in the native semen sample (before …
This test determines the maturity of the sperm in the native semen sample (before …
Link to the news
Thanks to technology and educated staff, we are able to treat even the most complicated cases of infertility, and thus increase the number of successful pregnancies resulting from medical insemination.
Following the new technological achievements, in the laboratory for medically assisted fertilization we use the most modern technologies and procedures in medical insemination.
Vitrification
A method for storing ova and embryos in which the sample is immersed directly in liquid nitrogen at -196 ° C, which lead...
A method for storing ova and embryos in which the sample is immersed directly in liquid nitrogen at -196 ° C, which leads to the “glazing” of the liquid part of the cells, the so-called vitrification. With the introduction of this cryopreservation method, oocyte survival increased to 90-100%.
IMSI procedure
From the very beginning of the treatment of male infertility by micromanipulation methods one of the main problems is th...
From the very beginning of the treatment of male infertility by micromanipulation methods one of the main problems is the choice of sperm. At a magnification of 200 to 400 times, most sperm anomalies that are visible on conventional fixed. The application of this method in the clinic has led to the development of a modified variant of ICSI called IMSI (intracytoplasmic morphologically selected sperm injection) by which we select morphologically best sperm when performing ICSI.
Seed preparation by magnetic separation of cells with early signs of damage (MACS)
DNA fragmentation is one of the invisible signs of sperm decay. We cannot distinguish such sperm from others until we st...
DNA fragmentation is one of the invisible signs of sperm decay. We cannot distinguish such sperm from others until we stain them in a certain way which helps us to diagnose their increased proportion, but after that we cannot use the remaining healthy cells in therapy e.g. by insemination or in IVF procedure. This method increases the success of insemination and the quality of embryos in in vitro fertilization.
Clean room technology in our laboratory
By equipping a new laboratory, every detail has been carefully selected to increase the chances of pregnancy. The labora...
By equipping a new laboratory, every detail has been carefully selected to increase the chances of pregnancy. The laboratory is located in the middle of the new space, isolated from all other rooms by air lock, the walls are made of antibacterial panels used in clean room technology, and paints and adhesives used in construction are selected to contain minimal amounts toxic substances. The process of gamete and embryo culture is completely protected from external influences.
MicroTESE
Microsurgical testicular sperm extraction (microTESE) is a surgical procedure used to retrieve sperm from the seminifero...
Microsurgical testicular sperm extraction (microTESE) is a surgical procedure used to retrieve sperm from the seminiferous tubules of a male’s testes. It’s used for men with non-obstructive azoospermia, which is when a man cannot produce enough sperm to have a detectable amount in his semen — a common cause of male infertility.
Link to the news
EmbryoGlue
EmbryoGlue is a medium that closely resembles the environment in the womb at the time of implantation. Placing the embry...
EmbryoGlue is a medium that closely resembles the environment in the womb at the time of implantation. Placing the embryo in EmbryoGlue before transfer gives it an even better chance to implant.
Link to the news
Laser Saturn 5 active (Laser assisted embryo hatching)
Assisted hatching is generally performed on the third day of embryo development. Our embryologists use state of the art ...
Assisted hatching is generally performed on the third day of embryo development. Our embryologists use state of the art laser (Laser Saturn 5 active) to create a very small hole in the zona pellucida. Assisted hatching can also be done on previously frozen and thawed embryos.
ZyMōt Fertility Sperm Separation Device
The ZyMōt multi sperm separation device prepares motile sperm from semen for use in intrauterine insemination (IUI) and ...
The ZyMōt multi sperm separation device prepares motile sperm from semen for use in intrauterine insemination (IUI) and intracytoplasmic sperm injection (ICSI) procedures. ZyMōt devices separate sperm based on motility, without the need for any centrifugation
PICSI Procedure
The method of sperm selection for the micromanipulation procedure is based on the ability …
The method of sperm selection for the micromanipulation procedure is based on the ability …
MAR Includes
Diagnosis and Treatment of Infertility
Today, it is estimated that about 15% have fertility problems in one or both partners. According to the instructions of the WHO (World Health Organization), if after one year of trying the desired pregnancy did not occur, you should inform your doctor for a consultation and preliminary tests
- Cervical swabs
- Induction of ovulation
- Hormonal status
- 3D ultrasound of uterus
- Spermiogram
- Insemintaion (AIH)
- Ultrasound 3D folliculometry
- Hysterosalpingo-foam sonography (HyFoSy)
In vitro fertilization (IVF)
Thanks to experience and equipment, we can treat even the most complicated cases of infertility in the polyclinic. Our first goal is to determine why you are not pregnant and then help you find the best and fastest solution to your problem. Every infertility is unique and should be approached that way.
How IVF works?
From the beginning of infertility treatment, it is important to solve the problem in a certain order, preferably diagnose the cause, and then treat it with the optimal method.
STEP 1
Recognize problem
If you do not get pregnant after having unprotected sex for 1 year. If you are older than 35 or if you do not get your p...
If you do not get pregnant after having unprotected sex for 1 year. If you are older than 35 or if you do not get your period every month, see your gynaecologist if you can’t get pregnant after 6 months of trying.
STEP 2
Consultations
From the beginning of infertility treatment, it is important to solve the problem in a certain order, preferably diagnos...
From the beginning of infertility treatment, it is important to solve the problem in a certain order, preferably diagnose the cause, and then treat it with the optimal method. Spermiogram, microbiological tests, gynecological and ultrasound examination are the basics from which to start.
STEP 3
Decision to go for IVF
After thorough preparations, when all the simpler ways of infertility therapy have been tried and it is established that...
After thorough preparations, when all the simpler ways of infertility therapy have been tried and it is established that in vitro fertilization is the only way you can get pregnant and give birth to a healthy child, we plan an appropriate assisted fertilization procedure. The procedure consists of the following stages.
STEP 4
Hormone stimulation
Based on the results of the diagnosis and basal values of pituitary hormones (2nd – 4th day of the cycle – F...
Based on the results of the diagnosis and basal values of pituitary hormones (2nd – 4th day of the cycle – FSH, LH, PRL), a stimulation protocol will be determined that will give the optimal result in your case.
STEP 5
Egg pickup
After the growth and maturation of the follicles, the follicular fluid in which the eggs are located is taken (aspirated...
After the growth and maturation of the follicles, the follicular fluid in which the eggs are located is taken (aspirated) by transvaginal puncture. In the IVF laboratory, the contents of the follicles are examined under a magnifying glass and eggs are isolated.
STEP 6
Fertilization
On the day of aspiration the partner gives semen which is processed and prepared for one of the IVF procedures. Dependin...
On the day of aspiration the partner gives semen which is processed and prepared for one of the IVF procedures. Depending on the finding, fertilization will be attempted by insemination of ova (in case of normozoospermia) or by ICSI (intracytoplasmic sperm injection) method in which sperm is selected, activated and inserted into a ovum previously prepared for this procedure with a glass micropipette.
STEP 7
Embriotransfer
On the second or fifth day after the puncture, depending on the number and quality of embryos, embryo transfer of one or...
On the second or fifth day after the puncture, depending on the number and quality of embryos, embryo transfer of one or more embryos is performed. How many embryos will return to the uterus depends on their quality, the number of previous attempts and the patient’s health.
Male fertility analysis
Infertility in a couple is defined as the inability to achieve conception despite one year of frequent, unprotected intercourse. In approximately 35 percent of couples with infertility, a male factor is identified along with a female factor.
When analyzing male fertility (spermiogram), among other things, we are able to make the following tests, analyzes and procedures.
Spermiogram
Spermiogram is an ejaculate analysis that determines the total number, concentration, mobility and appearance (morpholog...
Spermiogram is an ejaculate analysis that determines the total number, concentration, mobility and appearance (morphology) of sperm. In the diagnosis of male infertility, this is the basic examination on the basis of which the further course of action in the treatment of the infertility couple is decided.
More about spermiogram
SCA morphology
Is a program that allows automatic morphometric analysis and classification of sperm according to …
Is a program that allows automatic morphometric analysis and classification of sperm according to …
Link to the news
Halosperm – DNA fragmentation test
To determine which pairs are suitable for AIH, whether a modified approach (PICSI…
To determine which pairs are suitable for AIH, whether a modified approach (PICSI…
Link to the news
HBA Test
This test determines the maturity of the sperm in the native semen sample (before …
This test determines the maturity of the sperm in the native semen sample (before …
Link to the news
New Technologies
Thanks to technology and educated staff, we are able to treat even the most complicated cases of infertility, and thus increase the number of successful pregnancies resulting from medical insemination.
Following the new technological achievements, in the laboratory for medically assisted fertilization we use the most modern technologies and procedures in medical insemination.
Vitrification
A method for storing ova and embryos in which the sample is immersed directly in liquid nitrogen at -196 ° C, which lead...
A method for storing ova and embryos in which the sample is immersed directly in liquid nitrogen at -196 ° C, which leads to the “glazing” of the liquid part of the cells, the so-called vitrification. With the introduction of this cryopreservation method, oocyte survival increased to 90-100%.
IMSI procedure
From the very beginning of the treatment of male infertility by micromanipulation methods one of the main problems is th...
From the very beginning of the treatment of male infertility by micromanipulation methods one of the main problems is the choice of sperm. At a magnification of 200 to 400 times, most sperm anomalies that are visible on conventional fixed. The application of this method in the clinic has led to the development of a modified variant of ICSI called IMSI (intracytoplasmic morphologically selected sperm injection) by which we select morphologically best sperm when performing ICSI.
Seed preparation by magnetic separation of cells with early signs of damage (MACS)
DNA fragmentation is one of the invisible signs of sperm decay. We cannot distinguish such sperm from others until we st...
DNA fragmentation is one of the invisible signs of sperm decay. We cannot distinguish such sperm from others until we stain them in a certain way which helps us to diagnose their increased proportion, but after that we cannot use the remaining healthy cells in therapy e.g. by insemination or in IVF procedure. This method increases the success of insemination and the quality of embryos in in vitro fertilization.
Clean room technology in our laboratory
By equipping a new laboratory, every detail has been carefully selected to increase the chances of pregnancy. The labora...
By equipping a new laboratory, every detail has been carefully selected to increase the chances of pregnancy. The laboratory is located in the middle of the new space, isolated from all other rooms by air lock, the walls are made of antibacterial panels used in clean room technology, and paints and adhesives used in construction are selected to contain minimal amounts toxic substances. The process of gamete and embryo culture is completely protected from external influences.
MicroTESE
Microsurgical testicular sperm extraction (microTESE) is a surgical procedure used to retrieve sperm from the seminifero...
Microsurgical testicular sperm extraction (microTESE) is a surgical procedure used to retrieve sperm from the seminiferous tubules of a male’s testes. It’s used for men with non-obstructive azoospermia, which is when a man cannot produce enough sperm to have a detectable amount in his semen — a common cause of male infertility.
Link to the news
EmbryoGlue
EmbryoGlue is a medium that closely resembles the environment in the womb at the time of implantation. Placing the embry...
EmbryoGlue is a medium that closely resembles the environment in the womb at the time of implantation. Placing the embryo in EmbryoGlue before transfer gives it an even better chance to implant.
Link to the news
Laser Saturn 5 active (Laser assisted embryo hatching)
Assisted hatching is generally performed on the third day of embryo development. Our embryologists use state of the art ...
Assisted hatching is generally performed on the third day of embryo development. Our embryologists use state of the art laser (Laser Saturn 5 active) to create a very small hole in the zona pellucida. Assisted hatching can also be done on previously frozen and thawed embryos.
ZyMōt Fertility Sperm Separation Device
The ZyMōt multi sperm separation device prepares motile sperm from semen for use in intrauterine insemination (IUI) and ...
The ZyMōt multi sperm separation device prepares motile sperm from semen for use in intrauterine insemination (IUI) and intracytoplasmic sperm injection (ICSI) procedures. ZyMōt devices separate sperm based on motility, without the need for any centrifugation
PICSI Procedure
The method of sperm selection for the micromanipulation procedure is based on the ability …
The method of sperm selection for the micromanipulation procedure is based on the ability …
Our Happy Patients
Polyclinic CITO was declared the leader
in the number and percentage of success of medically assisted reproduction in the Republic of Croatia
Read News
Our Expert Doctors
Doc.Dr.sc. Nađa Aračić, MD
specialist in gynecology and obstetrics
subspecialist in gynecological endocrinology and human reproduction
dr.aracic@cito.hr
Petar Poljak, MD
specialist in gynecology and obstetrics
subspecialist in gynecological endocrinology and human reproduction
dr.petar.poljak@cito.hr
Primarius mr.sc. Boris Poljak, MD
specialist in Gynecology and obstetrics
Subspecialist in Gynecological endocrinology and human reproduction
dr.poljak@cito.hr
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